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AutoTune / SmoothQuick 'data' is stored permanently.  Once you have run AutoTune once, you can use it SmoothQuick with this stored data to produce smoother or quicker PIDs any time in the future.  You don't have to run AutoTune again, but you may want to if you change something, like props or weight or motors or cell count.

Using SmoothQuick semi-permanently:
If you enable the AutoTune module manually then it will always be running, even if there is no AutoTune flight mode or SystemIdent stabilization mode in your FMS.  In that case your SmoothQuick Accessory knob is always active for the destination PID bank (default 2) and you can adjust PIDs from smooth to quick any time you need it.

AutoTune data:
The settings used by SmoothQuick are all stored permanently in SystemIdentSettings.  They are Tau and the 3 Beta values (for the temporary LP-340 version, there is also GyroReadTimeAverage).  'Complete' must also be True.  You can copy these by hand from another FC or from a previous tuning session.  Then while disarmed and depending on which kind of SmoothQuick input you have enabled, you should do the SmoothQuick FMS 3x double toggle or move (important) and center your SmoothQuick Accessory channel with everything powered up (e.g. flight battery plugged in for the receiver and transmitter switched on).  This last bit will guarantee that you start out with PIDs in the middle of the SmoothQuick range.

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